Documentation

Safety Data Sheet	X-Gluc DIRECT SDS 4.0 2023
Certificate of Analysis	X-Gluc DIRECT COA P5-529-114
Certificate of Analysis	X-Gluc DIRECT COA P5-494-073
Certificate of Analysis	X-Gluc DIRECT COA P5-478-013

About X-Gluc

X-Gluc has gained international acceptance as an accurate indicator of the presence of E. Coli in several practical applications. It reduces false positives and negatives found with other methods.

X-Gluc is a chromogenic substrate used to detect β-glucuronidase, an enzyme produced by the E. Coli bacterium. Upon reduction, X-Gluc produces a localised colour, making it useful in identifying GUS gene presence in most cell types and detecting the GUS gene fusion marker in plants.

Applications and Uses of X-Gluc

X-Gluc, or 5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, is commonly used in molecular biology and biochemistry research to detect and quantify β-glucuronidase (GUS) activity. Here are some of the primary applications and uses of X-Gluc:

• Reporter Gene Assays: X-Gluc is used as a substrate to assess the activity of the GUS reporter gene. GUS is used in molecular biology, where X-Gluc allows researchers to monitor its activity. This is useful in studying gene expression, promoter activity, and the effects of regulatory elements on gene transcription.
• Gene Expression Studies: X-Gluc is used to visualise and quantify gene expression patterns. Researchers fuse the GUS reporter gene to a gene of interest and then monitor GUS activity as a proxy for the expression of that gene. This is performed in plant biology research to study the spatial and temporal patterns of gene expression.
• Promoter Analysis: X-Gluc is used to assess the strength and specificity of gene promoters. By placing the GUS reporter gene under the control of different promoters and measuring GUS activity in response to various conditions, researchers gain insights into how promoters function and respond to regulatory factors.
• Monitoring Transgenic Organisms: In plant and microbial research, X-Gluc identifies and tracks transgenic organisms that have been genetically modified to express GUS. This is important for studying the behavior of genetically modified organisms in different environments.
• Quantitative Assays: X-Gluc assays are quantified spectrophotometrically or using other methods, allowing for precise measurement of GUS activity. This quantitative data enables comparative analysis and statistical assessments.
• Environmental Microbiology: X-Gluc detects and quantifies GUS-tagged micro-organisms in environmental samples, which is useful for studying microbial ecology, bioremediation, and other environmental processes.
• Educational and Outreach Activities: X-Gluc is used in educational settings to demonstrate enzymatic reactions and the principles of molecular biology. Its blue colour reaction product is visually striking and engaging for students.

X-Gluc is a valuable tool for researchers in various fields, including plant biology, microbiology, genetics, and molecular biology. It enables the detection and measurement of GUS activity, which serves as a proxy for gene expression and promoter activity.

X-Gluc Details

• Product: 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt
• Molecular Formula: C₂₀H₂₆BrClN₂O₇
• Molecular Weight: 521.80
• CAS No: 114162-64-0
• Appearance: Crystalline white powder
• Solution: Clear solution when dissolved in DMF (5mg/ml)
• High purity research grade: >99%

Molecular Structure